Plus reactions worked differently: they used T4 DNA polymerase, an enzyme with strong 3’ to 5’ exonuclease activity, meaning it can chew back the end of a DNA strand. In the presence of only one dNTP, T4 DNA polymerase would degrade each fragment from its 3’ end until it reached a nucleotide complementary to that dNTP, at which point the exonuclease activity would be inhibited. This ensured that all fragments in a given plus reaction ended with the same nucleotide.
│ EXPECTED IMPACT: 5-15% recall improvement in specialized subsystems. │
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我们党历来反对形式主义。早在革命战争年代，毛泽东同志就批评形式主义是一种“幼稚的、低级的、庸俗的、不用脑筋”的东西。习近平总书记指出：“形式主义、官僚主义是实现新时代新征程党的使命任务的大敌，是党内的一个顽瘴痼疾。”形式主义的背后，往往是功利主义、实用主义作祟，根源在于政绩观错位、责任心缺失。