Real-mode programs freely execute CLI and STI to control interrupts, PUSHF and POPF to manipulate flags, INT n for DOS and BIOS calls, and IN/OUT for hardware I/O. In normal protected mode, these instructions are privilege-checked -- they execute normally if the caller has sufficient privilege, and fault otherwise. The 386 can't simply let V86 tasks execute them freely -- a DOS program disabling interrupts would bring down the whole system -- but trapping on every INT 21h call would make V86 impractically slow.
The bigger your RAM, the bigger your detector, so use a desktop's RAM if you can
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